| 引物名称 | 序列(5’to3′) | 碱基数(mer) |
|---|---|---|
| M13F(-77) | GATGTGCTGCAAGGCGATTA | 20 |
| M13R(-88) | TTATGCTTCCGGCTCGTATG | 20 |
| M13F(-47) | CGCCAGGGTTTTCCCAGTCACGAC | 24 |
| M13R(-48) | AGCGGATAACAATTTCACACAGGA | 24 |
| T7 | TAATACGACTCACTATAGGG | 20 |
| T7t | TGCTAGTTATTGCTCAGCGG | 20 |
| SP6 | ATTTAGGTGACACTATAG | 18 |
| T3 | ATTAACCCTCACTAAAGGGA | 20 |
| pGEX-5 | GGGCTGGCAAGCCACGTTTGGTG | 23 |
| pGEX-3 | CCGGGAGCTGCATGTGTCAGAGG | 23 |
| pEGFP-N-5 | TGGGAGGTCTATATAAGCAGAG | 22 |
| pEGFP-N-3 | CGTCGCCGTCCAGCTCGACCAG | 23 |
| CMV-Forward | CGCAAATGGGCGGTAGGCGTG | 21 |
| U6-promotor | ATGGACTATCATATGCTTACCGTA | 24 |
| pEGFP-N-3’ | CGTCGCCGTCCAGCTCGACCAG | 22 |
| pEGFP-N-5’ | TGGGAGGTCTATATAAGCAGAG | 22 |
| pEGFP-C-3’ | TATGGCTGATTATGATCAGT | 20 |
| pEGFP-C-5’ | CATGGTCCTGCTGGAGTTCGTG | 22 |
| pFastBac-F | GGATTATTCATACCGTCCCA | 20 |
| pFastBac-R | CAAATGTGGTATGGCTGATT | 20 |
| 3’AD | AGATGGTGCACGATGCACAG | 20 |
| BGHR | TAGAAGGCACAGTCGAGG | 18 |
| S-Tag | CGAACGCCAGCACATGGACA | 20 |
| RVP4 | GACGATAGTCATGCCCCGCG | 20 |
| pQE30-R | GTTCTGAGGTCATTACTGG | 19 |
| pQE30-F | TGAGCGGATAACAATTTCAC | 21 |
| 5-AOX1 | GACTGGTTCCAATTGACAAGC | 21 |
| 3-AOX1 | GCAAATGGCATTCTGACATCC | 21 |
| RSFDuet-1-seqF | CATCACCATCATCACCACAGCCAGG | 25 |
| pTrcHis-F | GAGGTATATATTAATGTATCG | 21 |
| pTrcHis-R | GATTTAATCTGTATCAGG | 18 |
| M13F/M13(-21) | TGTAAAACGACGGCCAGT | 18 |
| M13R | CAGGAAACAGCTATGAC | 17 |
| 27F | AGAGTTTGATCCTGGCTCA | 19 |
| 1492R | GGTTACCTTGTTACGACTT | 19 |
| EF3 | TCCTCTAAATGACCAAGTTTG | 21 |
| EF4 | GGAAGGGRTGTATTTATTAG | 20 |
| NS1 | GTAGTCATATGCTTGTCTC | 19 |
| NS8 | TCCGCAGGTTCACCTACGGA | 20 |
| ITS4 | TCCTCCGCTTATTGATATGC | 20 |
| ITS5 | GGAAGTAAAAGTCGTAACAAGG | 22 |
| ITS1 | GTAGGTGAACCTGCGG | 16 |
| NL1 | GCATATCAATAAGCGGAGGAAAAG | 24 |
| NL4 | GGTCCGTGTTTCAAGACGG | 19 |
| pCold-F | ACGCCATATCGCCGAAAGG | 19 |
| pCold-R | GGCAGGGATCTTAGATTCTG | 20 |
| v1.5 | GGACTT TCCAAA ATG TCG | 22 |
| XL39 | ATTAGGACAAGGCTGGTGGG | 20 |
| RVP3 | CTAGCAAAATAGGCTGTCCC | 20 |
| EF1A-F | TCAAGCCTCAGACAGTGGTTC | 21 |
| pPIC9K-seqR | ATGGCATTCTGACATCCTCTT | 21 |
| a-factor | TACTATTGCCAGCATTGCTGC | 21 |
| 3BD | TAAGAGTCACTTTAAAATTTGTATC | 25 |
| 5AD | AGGGATGTTTAATACCACTAC | 21 |
| PCMV-F | TCTAAAAGCTGCGGAATTGT | 20 |
| PCMV-R | TCCAAACTCATCAATGTATC | 20 |
| pBABE-3 | ACCCTAACTGACACACATTCC | 21 |
| pBABE-5 | CTTTATCCAGCCCTCAC | 17 |
| pBAD-Forward | ATGCCATAGCATTTTTATCC | 20 |
| pBAD-Reverse | GATTTAATCTGTATCAGG | 18 |
| PBV220F | AAGAAGGGCAGCATTCAAAG | 20 |
| PBV220R | CTGCGTTCTGATTTAATCTG | 20 |
| GLP2 | CTTTATGTTTTTGGCGTCTTCCA | 23 |
| PIRES3-EGFP-R | AACGCACACCGGCCTTATTC | 20 |
| PIRES-F | CTTACTGACATCCACTTTGC | 20 |
| PIRES-R | CACTGCATTCTAGTTGTGGT | 20 |
| Human-U6 | GGGCAGGAAGAGGGCCTA | 18 |
| DuetUp2 | TTGTACACGGCCGCATAATC | 20 |
| DuetDown1 | GATTATGCGGCCGTGTACAA | 20 |